Repligen chromatography systems and expert consultancy can bring increased process recovery and operational efficiencies to even the most complex new modalities.
increase recOvery
Reduce in-line turbulence and gradient dilution for improved resolution and recovery
maintain bioactivity
Valve blocks and injection molded tubing junctions help reduce mechanical stress to the product
reduce risk of deviations
Pre-assembled flow kits and simpler batch changes reduce human error and contamination events
Decades of component and system evolution
Increase recovery by protecting gradient integrity and peak resolution
Maintaining gradient control from the mixing valve to the column and protecting peak resolution is critical to maximizing recovery, but can be challenging with traditional systems. Dilution, turbulence and dead-legs can all occur along the flowpath and disrupt the process. Repligen has developed technologies specifically targeted to eliminate or minimize those challenges.
Protect gradient accuracy by minimizing dilution
Traditional bubble trap
Up to 500ml of hold-up volume can uncontrollably disrupt gradient, causing back mixing and dilution, harming reproducibility and recovery.
Repligen filter and level sensor solution
- Use of a filter minimizes hold up volume helps maintain gradient control and promotes higher recovery
- A level sensor coupled with the filter enables the automation of air release
Reduce gradient disruption by removing dead legs
Traditional valve solutions at tubing junctions
- Individual valves spaced far apart due to the use of hose-barbs create significant dead legs
- Dead legs can trap sample, cause cross-contamination and create turbulence that compromises both gradient integrity and peak resolution post-column
Valve block technology
- Valve blocks bring the pinch points right to the main line, shortening the flow path and eliminating dead legs
- With no dead-legs to disrupt flow dynamics, the gradient going into the column and peaks coming off can remain consistent and
Protect product integrity from shear damage
Traditional technology
Traditional solutions utilize hose barbs, zip ties and individual valves at tubing junctions causing excess shear stress to the product as inner diameter narrows and flow dips into significant dead legs.
Repligen Technology
The consistent ID of overmolded connections coupled with injection molded tubing and valve block hardware at critical junctions eliminate dead-legs, minimize shear stress and protect product integrity.
Simplify operations and reduce risk with a better user experience
Repligen workflow
Repligen has simplified user experience and minimized risk during batch changes reducing equipment downtime and saving valuable resources.
Receive
Flow path arrives in four gamma irradiated sub-assemblies.
Install
- Pre-calibrated sensors
- 3 connections aseptic/hosebarb
- Mistake-free installation
Run
Ready to go
Flow path installation on a ¼ inch system
Traditional workflow
Batch change on traditional single-use chromatogrpahy systems can be resource intensive, require hours, tie up more than one person and necessitate many more manual interactions during installation increasing overall risk.
Increase operational efficiencies with a complete solution
Repligen has developed or acquired multiple chromatography technologies that in conjunction with our systems work to further increase operational efficiencies for your process and facility.
リソース
|
1/4” ID |
1/2” ID |
3/4” ID |
内径1インチ |
|
| 流量 | 1 - 180 L/h | 6 - 900 L/h | 25 - 2000 L/h | 50 - 3600 L/h |
| Column size | 8 - 25 cm ID | 20 - 45 cm ID | 45 - 80 cm ID | 60 - 80 cm ID |
| Pump type | 2 Quattroflow™ pumps | |||
| センサー |
2 flow meters |
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| ソフトウェア | AB PLC and Wonderware | |||
| Multiple flow paths | One built for purpose flowpath on each system for optimal process efficiency | |||