KrosFlo^® KPS TFF Systems

For use in pilot to large-scale perfusion cell culture, the KrosFlo^® KPS Systems are integrated tangential flow filtration (TFF) systems.
     • 500L - 5000L volume 

TFF perfusion for commercial production

The KrosFlo^® KPS Systems automatically control and maintain critical operational parameters defined by the operator during pilot and large-scale cell culture perfusion. While traditional stainless steel systems need to be cleaned in place (CIP) or steamed in place (SIP) prior to use, the KrosFlo^® KPS Systems are integrated with sterile ProConnex Flow Paths for easy ready-to-operate tangential flow perfusion. 

 

        How hollow fiber TFF Works

KrosFlo^® KPS TFF SYSTEMS

KrosFlo^® KPS Systems are set up on a mobile cart that supports the hollow fiber filter module,  an integrated ProConnex^® flow path, and an integrated TFF system that automatically controls and maintains critical operational parameters (Recirculation Shear Rate, TMP control, Permeate Flow/VVD and Reactor Volume Maintenance). Robust integrated solutions and associated connections minimize the hold up volume and provides a completely drainable process loop.

Integrated system

Hollow fiber filter, configurable ProConnex^® flow path, complete TFF system mounted on a mobile cart

custom flow path

Fully integrated with sterile,custom-engineered ProConnex^® flow path

スケーラブル

Compatible with KrosFlo^® TFF perfusion systems from process development to commercial manufacturing

Automatic Back Pressure Control Valve

Unique to Repligen, the KrosFlo^® Back Pressure Control Valve is key to integrating and automating the TFF process, reducing operator process management time and TFF processing time.

• Automatically adjusts tubing pinch distance according to user-defined set-points for inlet, retentate, permeate or transmembrane pressures (TMP) when attached to the ProConnex^® flow path
• Maintains a uniform TMP throughout the process run, decreasing membrane fouling
• Controls the permeate pressure and flow during microfiltration applications
• Releases and tightens the pinch valve as solution viscosity increases or decreases during a process
• Can be set to start pinching from the open, middle, or closed position
• Compatible with tubing sizes 13, 14, 16, 25, HP15, 17, HP24, 18, HP35, 36, and HP36

CONFIGURABLE Pro-Connex^® FLOW PATHS

KF Comm Software

• Automatically collects process data
• Provides real-time overviews of the filtration process

Microsoft Surface Pro 4

• Automatically graphs data in Excel-based software
• 21CFR Part 11 enabled when used with optional KF Comm2 software package

Real-time operating parameters

• Time
• Feed Pressure
• Retentate Pressure
• Permeate Pressure
• Feed Flow Rate
• Diafiltration Flow Rate
• Permeate Flow Rate 
• Feed Scale Weight
• Permeate Scale Weight
• Pump RPM
• Notes (Alarms, Process status, Functions)
• Pump Mode

Processing parameters

• Transmembrane Pressure (TMP)
• Pressure Drop (ΔP)
• Retentate Flow Rate
• Permeate Flux
• Volumetric Throughput (VT)
• Concentration Factor
• Shear
• Diafiltration Volumes
• Process Time
• UV, Conductivity, and Temperature (with KONDUiT)

Compatible Filter Modules

See hollow fiber membrane types

Scalable from 500L - 5000l