シングルユースモジュールに完全にカプセル化された、中空糸フィルターモジュールで使用されるRepligenのメンブレンは、構造的異方性を有する4種類の化学組成で構築されています。高い流速、優れた分離、低いタンパク結合率を可能とし、生成物の収量を向上できます。
Ultrafiltration (UF) membranes have a high density skin layer for less fouling and a fairly open outer support structure for increased filtration rates.
Microfiltration (MF) membranes have a retention defining microporous outer skin and a more open inner layer for increased filtration with higher recoveries.
Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。
Membrane Types
With a broad choice of single-use module configurations, hollow filter membranes from Repligen use advanced hydrophilic membrane filtration chemistry to provide higher flux rates for faster processing times, excellent selectivity for separation applications, and low protein binding for higher product yields than other membrane chemistries.
| メンブレン | Fiber internal diameter | MWCO and Pore Size | # Module Configurations | # Effective Lengths | |
|---|---|---|---|---|---|
| mPES | Modified Polyethersulfone | 0.50 mm | 1kD, 3kD, 5kD, 10kD, 30kD, 50kD, 70kD, 100kD, 300kD, 500kD, 750kD | 7 | 3 |
| mPES | Modified Polyethersulfone | 0.75 mm | 0.65µm | 7 | 3 |
| mPES | Modified Polyethersulfone | 1.00 mm | 3kD, 5kD, 10kD 30kD, 50kD, 70kD, 100kD, 300kD, 500kD, 750kD | 7 | 3 |
| ME | Mixed Cellulose Ester | 0.63 mm | 0.1 µm、0.2 µm | 7 | 3 |
| ME | Mixed Cellulose Ester | 1.00 mm | 0.2µm | 7 | 3 |
| PS | Polysulfone | 0.5 mm | 10kD, 50kD, 500kD and 0.05µm | 7 | 3 |
| PES | Polyethersulfone | 0.5 mm | 0.2µm | 7 | 3 |
| PES | Polyethersulfone | 1.0 mm | 0.2µm | 7 | 3 |
Membrane Applications
以下の表は、メンブレン特性の一般的な使用方法を、用途別に示したものです。
| 用途 | Membrane selection | mPES | ME | PS | PES |
|---|---|---|---|---|---|
| Concentration and diafiltration (buffer exchange) of proteins, peptides, enzymes, recombinant proteins | 1, 3, 5, 10, 30, 50, 70,100kD | x | x | ||
| Depyrogenation of water / buffers | 3, 10kD | x | x | ||
| Coating, diafiltration, fractionation and/or dewatering of nanoparticles | 70,100,300,500,750kD, 0.2µm | x | x | ||
| Concentration and diafiltration of albumin (nominal MW 67kd) | 10kD | x | |||
| Concentration and diafiltration of monoclonal antibodies (155kd) | 30kD | x | |||
| Concentration and diafiltration of envelope and non-envelope virus | 50, 70, 100, 300, 500, 750kD, 0.05µm | x | x | ||
| Clarification of cell lysates, viral-expressed cultures to recover product in permeate | 500, 750kD, 0.05, 0.1µm | x | x | x | |
| Concentration and washing of insect, bacterial and/or mammalian cells |
750 kD、0.1, 0.2, 0.65 µm | x | x | ||
| Continuous bioreactor perfusion of cell culture | 30, 50, 70, 100, 300, 500, 750kD, 0.2, 0.65µm | x | x | x | |
| Concentration and diafiltration of single-stranded or double-stranded nucleic acids | 1, 3, 5, 10, 30, 50, 70,100kD | x | |||
| Washing latex, polystyrene and other diagnostic particles to remove un-reactives / unbound drug substance |
500, 750kD, 0.05, 0.1, 0.2, 0.65µm | x | x | x |