Dare to Innovate Accross the Workflow

Rediscover the latest bioprocessing innovations, presentations, and scientific posters unveiled during our 2024 tradeshows. Insights for intensifying your cell culture, optimizing your process development (PD), and accelerating your speed to the market are available throughout.

Lifesaving biologics from bioreactors to bedside. Fill out the form to uncover methods and studies that can enrich your process through one or all of these special presentations. 

Reducing risk in bioprocess manufacturing through innovation

Minimizing risk is paramount to ensure process efficiency and integrity of biotherapeutics. This talk will discuss innovative solutions Repligen has launched to enable customers to mitigate risk throughout their manufacturing processes, to enhance safety and efficiency of life changing therapeutics.      

Intensified Production of Recombinant Vesicular Stomatitis Virus-Based Vectors by Tangential Flow Depth Filtration

In this study, we evaluated the use of a tangential flow depth filtration (TFDF) unit for both perfusion cultivation and continuous harvesting with clarification in a single unit operation at the 3 L scale. The intensified production of two different rVSV-based vectors, one inducing classical cytopathic effect and one mediating cell fusion reactions, was compared to optimized batch control processes. 

Using HEK293-SF cells and a recombinant vesicular stomatitis virus (rVSV) vector expressing a green fluorescent protein, perfusion cultivations resulted in a maximum viable cell concentration (VCC) of 11.3x106 cells/mL and infectious virus titers up to 7.1x1010 TCID50/mL in the permeate. Integrated continuous clarification during the virus production was possible. Although the cell-specific virus yield decreased relative to a batch control process, an increased space-time yield was obtained. Using suspension BHK-21 cells and a fusogenic oncolytic hybrid of rVSV and Newcastle disease virus (rVSV-NDV), the integrated TFDF technology allowed to achieve high VCCs (16.4 20.6x106 cells/mL), continuous vector harvesting and clarification. Compared to an optimized batch process, 11-fold higher infectious virus titers were obtained in the permeate (maximum 7.5x109 TCID50/mL), resulting in a 460% increase in space-time yield.

Latest Innovations

KrosFlo RS 10 TFF System

The first automated, bench-scale TFF system built specifically for cGMP production. The KrosFlo RS 10 tubing is an 1/8th-inch inner diameter and features the same components, controls, and capabilities as a large-scale TFF system used in commercial manufacturing. This includes all the valves, inlets, and outlets for complete end-to-end automation of the entire TFF process, such as buffer and caustic flushing, integrity and permeability testing, ultrafiltration and diafiltration (UF/DF), and product recovery.

TangenX^® SC 

The TangenX^® SC is a self-contained, single-use device featuring a true flat sheet design that is gamma sterilized. With no need for a holder or torquing, it helps users eliminate product loss risk, reduce set-up time by 80%, and eliminate decommission time, thereby enhancing facility efficiency.

ProConnex^® MixOne Carboy System

For those high-value bioprocess drug products where superior mixing, secure storage, or safe transportation of pharmaceutical products is critical, the ProConnex^® MixOne Carboy system offers industry leading mixing technology, Truelev, integrated into a secure single-use rigid carboy which can be easily and safely transported in the ProConnex^® MixOne Secure Carrier. 

ProConnex^® RG-V film 

Repligen ProConnex^® RG-V Film is a state-of-the-art film developed specifically to meet the stringent quality standards of upstream and downstream single-use bioprocessing applications. Used to manufacture standard and custom 2D and 3D bag assemblies, this film offers superior leak and puncture resistance, excellent flex crack resistance, and optimal gas and water vapor barrier properties.