Click and quote OPUS® PD Pre-packed chromatography columns


Configure and quote pre-packed columns using the latest web-based technology.

When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes. 

Make your chromatography life easier.

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Flexibility with pre-packed convenience

  • ユーザー指定の300種類以上のクロマトグラフィー樹脂がプレパックされています
  • Consistently packed with correct axial compression
  • 液体処理ワークステーションの分注速度によって制御される直線流量制御
  • 一定の滞留時間によるスケールアップ、スケールダウンを実証済み
  • 一度に多数のサンプル、マルチプレックス対応



cm mm cm mm ml µL
0.5 5.0 0.25 2.5 0.05 50
0.5 5.0 0.50 5.0 0.10 100
0.5 5.0 1.00 10.0 0.20 200
0.5 5.0 2.25 22.5 0.45 450
0.5 5.0 2.50 25.0 0.50 500
0.5 5.0 3.00 30.0 0.60 600

Minimum order is one row of 8 OPUS  RoboColumn pre-packed columns  with the same resin and column dimension.


Automated parallel chromatography

Shorten process development time significantly by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design).

Designed for robotic fluid handlers 

Compatible with both Tecan and PerkinElmer platforms, the preferred supplier for OPUS RoboColumn automation is the Tecan Freedom EVO® robotic workstation.

  • 96ウェルプレートへの同時自動ステップ勾配溶離
  • 統合されたプレートリーダーによるその後の分析
  • 柔軟なソフトウェアウィザード 

The combination of OPUS RoboColumn and the Tecan Freedom EVO® robotic workstation delivers a complete integrated platform for state-of-the-art small-scale protein purification.

Rapid and simple AAV capture and polish DOE with new digital tools

This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan’s Fluent™ robotic workstation.

Configure your columns

再生アイコン 動画ポスター

 High Performance


  • 樹脂スクリーニング/条件スクリーニング

Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step. Using OPUS RoboColumn on a Tecan Freedom EVO® generated up to 96 pseudo-chromatograms (1536 data-points) in 1.5 days when comparing 12 CIEX resins at 8 different pH 3.7-7.0.

  • 16分でそれぞれ16のフラクションと並行して8つのクロマトグラフィー分離
  • 10分で96の個別バッファー


  • インプロセス発酵モニタリング
  • タンパク質特性評価
  • 創薬

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates.  Non-purified samples require sample preparation before analysis can be executed. OPUS RoboColumn enables one-step purification of multiple samples in parallel.

Using the OPUS RoboColumn on a Tecan Freedom EVO® generates 2mg to 24mg API (Active Pharmaceutical Ingredient) per RoboColumn® run.

OPUS RoboColumn Pre-packed Chromatography Colums

Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

OPUS® RoboColumn®
internal design

OPUS® RoboColumn®
96-well plate

OPUS® RoboColumn®
5 mm ID x 30 mm H;V = 600 µL

OPUS® RoboColumn®
5 mm ID x 10 mm H;V = 200 µL

Manufacturing Centers of Excellence

Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.




  • Field Application Support
  • カスタマーサービス
  • フィールドサービスエンジニア





128.3 L x 86.0W x 14.0 mm H






50 µL

100 µL

200 µL

450 µL

500 µL

600 µL


2.5 mm

5.0 mm

10.0 mm

22.5 mm

25.0 mm

30.0 mm

カラム内 直径

5 mm




一般的に使用されるすべての水性バッファー、pH 1〜14、有機溶剤**。ハロゲン化有機溶剤、ヘキサンは使用しないでください


AIECおよびCIECカラム: エタノール20% + 150 mM塩化ナトリウム
HIC、SEC、アフィニティ、および MMCカラム: エタノール20%
CHT、CFTカラム(Bio-Rad): エタノール20% + 10 mMリン酸ナトリウム、pH6.8
ProSeP®カラム(Millipore): ベンジルアルコール1% + 0.1M 酢酸ナトリウム、pH 5.1

推奨 保管温度

アフィニティカラム: +2°C~+8°C



最大動作 圧力



16~1000 cm/h**



  • 製品番号
  • 樹脂名
  • カラム容量(µL)

**Te‑Chrom™ WizardのLiquid Transferメニューでは、分注ごと、クロマトグラフィーのステップごとに流量を設定できます( Transfer、Te‑Chrom™ Wizard Software Manual、396076、en、V1.0).