OPUS RoboColumn Pre-packed Chromatography Colums
Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.
Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.
![96 well plate OPUS RoboColumn pre-packed chromatography column](https://www.repligen.com/Products/OPUS/OPUS%20RoboColumn_Plate_Intro_Start_Configurator_Shopping%20card%20%282%29.png)
OPUS® RoboColumn®
internal design
OPUS® RoboColumn®
5 mm ID x 10 mm H;V = 200 µL
OPUS® RoboColumn®
5 mm ID x 30 mm H;V = 600 µL
OPUS® RoboColumn®
96-well plate
![](https://www.repligen.com/Products/OPUS/OPUS_Lineup_13Feb2024.jpg)
Click and quote OPUS® PD Pre-packed chromatography columns
![homescreen of OPUS robocolumn configurator](https://www.repligen.com/_default_upload_bucket/get-image-thumbnail_51.png)
Configure and quote pre-packed columns for process development using this interactive tool.
When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes.
Make your chromatography life easier.
FLEXIBILITY
Flexibility with pre-packed convenience
- ユーザー指定の300種類以上のクロマトグラフィー樹脂がプレパックされています
- Consistently packed with correct axial compression
- 液体処理ワークステーションの分注速度によって制御される直線流量制御
- 一定の滞留時間によるスケールアップ、スケールダウンを実証済み
- 一度に多数のサンプル、マルチプレックス対応
利用可能なフォーマット
内径 |
ベッド高さ |
カラム容量 |
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cm | mm | cm | mm | ml | µL |
0.5 | 5.0 | 0.25 | 2.5 | 0.05 | 50 |
0.5 | 5.0 | 0.50 | 5.0 | 0.10 | 100 |
0.5 | 5.0 | 1.00 | 10.0 | 0.20 | 200 |
0.5 | 5.0 | 2.25 | 22.5 | 0.45 | 450 |
0.5 | 5.0 | 2.50 | 25.0 | 0.50 | 500 |
0.5 | 5.0 | 3.00 | 30.0 | 0.60 | 600 |
Minimum order is one row of 8 OPUS RoboColumn pre-packed columns with the same resin and column dimension.
Automation
Automated parallel chromatography
Shorten process development time by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design). The combination of OPUS RoboColumn and Tecan robotic liquid handling workstations deliver a complete integrated platform for state of the art small scale protein purification.
Designed for robotic fluid handlers
Compatitble with both Tecan Fluent and Freedom EVO liquid handling workstations
- 96ウェルプレートへの同時自動ステップ勾配溶離
- 統合されたプレートリーダーによるその後の分析
- 柔軟なソフトウェアウィザード
![](https://www.repligen.com/Products/OPUS/Fluent_780_Te-Chrome_V1_01%201%20%282%29.png)
Rapid and simple AAV capture and polish DOE with new digital tools
This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan Fluent liquid handling workstation.
![動画ポスター](https://img.youtube.com/vi/qDtpvn2rIb8/0.jpg)
High Performance
プロセス開発![](https://www.repligen.com/Products/OPUS/OPUS_Diagram01 (2).jpg)
Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step.
- Resin screening
- Condition screening
Using OPUS RoboColumn on a Tecan Fluent liquid handling workstation generated up to 96 pseudo-chromatograms (1536 data-points) in less than one day.
サンプル調製/プロセス分析技術(PAT)
The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples derived from small scale multi-parallel bioreactors, such as Ambr™, requires sample preparation before analysis can be executed.
- Drug discovery and clone selection
- タンパク質特性評価
- Media optimization
- インプロセス発酵モニタリング
Using the OPUS RoboColumn on a Tecan Fluent liquid handling workstation generates sufficient purified product to conduct your analytical assays.
Manufacturing Centers of Excellence
Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。
Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.
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お客様第一。
サポートは、Repligenという企業の遺伝子に組み込まれています。弊社の目標は、卓越した顧客体験を提供すること、そしてRepligenの製品やサービスの適用や導入を効率よく成功に導くためにサポートすることです。
- Field Application Support
- カスタマーサービス
- フィールドサービスエンジニア
リソース
仕様
プレートのサイズ |
128.3 L x 86.0W x 14.0 mm H |
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プレートの素材 |
ポリオキシメチレン共重合体 |
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穴の数 |
96 |
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カラム容量 |
50 µL |
100 µL |
200 µL |
450 µL |
500 µL |
600 µL |
ベッド高さ |
2.5 mm |
5.0 mm |
10.0 mm |
22.5 mm |
25.0 mm |
30.0 mm |
カラム内 直径 |
5 mm |
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カラムの素材 |
ポリプロピレン |
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化学的安定性 |
一般的に使用されるすべての水性バッファー、pH 1〜14、有機溶剤**。ハロゲン化有機溶剤、ヘキサンは使用しないでください |
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カラム保存溶媒 |
AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride |
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推奨 保管温度 |
AIEC、CIEC、HIC、SEC、MMC: +4°C~+30°C |
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動作温度 |
+4°C~+30°C |
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最大動作 圧力 |
最大8.0bar |
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流速 |
16~1000 cm/h** |
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ラベリング |
各カラム本体にレーザーエッチング。ラベルの内容:
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*注:充填したクロマトグラフィー樹脂の化学的安定性も考慮する必要があります。詳細については、メーカーの樹脂仕様を参照してください。