OPUS® RoboColumn® Pre-packed Columns

LEADER OF THE PACK

OPUS^®RoboColumn^® miniaturized pre-packed columns are designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.

Why it matters Repligenについて関連製品リソース仕様樹脂の一覧

見積を請求

Flexibility

Pre-packed column convenience

User-specified chromatography resin

Multiple column volumes

Automation

Use with Tecan robotic fluid handlers

Configuration your column your way

高性能

Proven resin screening applications

Ideal for sample preparation and PAT

Linearly scalable to other OPUS columns

OPUS RoboColumn Pre-packed Chromatography Colums

Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.

Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.

96 well plate OPUS RoboColumn pre-packed chromatography column

OPUS^® RoboColumn^®
internal design

OPUS^® RoboColumn^®
5 mm ID x 10 mm H；V = 200 µL

OPUS^® RoboColumn^®
5 mm ID x 30 mm H；V = 600 µL

OPUS^® RoboColumn^®
96-well plate

Click and quote OPUS^® PD Pre-packed chromatography columns

homescreen of OPUS robocolumn configurator

Configure and quote pre-packed columns for process development using this interactive tool.

When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes.

Make your chromatography life easier.

Get started

FLEXIBILITY

Flexibility with pre-packed convenience

ユーザー指定の300種類以上のクロマトグラフィー樹脂がプレパックされています
Consistently packed with correct axial compression
液体処理ワークステーションの分注速度によって制御される直線流量制御
一定の滞留時間によるスケールアップ、スケールダウンを実証済み
一度に多数のサンプル、マルチプレックス対応

樹脂の一覧を見る

利用可能なフォーマット

内径		ベッド高さ		カラム容量
cm	mm	cm	mm	ml	µL
0.5	5.0	0.25	2.5	0.05	50
0.5	5.0	0.50	5.0	0.10	100
0.5	5.0	1.00	10.0	0.20	200
0.5	5.0	2.25	22.5	0.45	450
0.5	5.0	2.50	25.0	0.50	500
0.5	5.0	3.00	30.0	0.60	600

_{Minimum order is one row of 8 OPUS RoboColumnpre-packed columns with the same resin and column dimension.}

Automation

Automated parallel chromatography

Shorten process development time by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design). The combination of OPUS RoboColumn and Tecan robotic liquid handling workstations deliver a complete integrated platform for state of the art small scale protein purification.

Designed for robotic fluid handlers

Compatitble with both Tecan Fluent and Freedom EVO liquid handling workstations

96ウェルプレートへの同時自動ステップ勾配溶離
統合されたプレートリーダーによるその後の分析
柔軟なソフトウェアウィザード

Rapid and simple AAV capture and polish DOE with new digital tools

This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan Fluent liquid handling workstation.

High Performance

プロセス開発

Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step.

Resin screening
Condition screening

Using OPUS RoboColumn on a Tecan Fluent liquid handling workstation generated up to 96 pseudo-chromatograms (1536 data-points) in less than one day.

サンプル調製/プロセス分析技術（PAT）

The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples derived from small scale multi-parallel bioreactors, such as Ambr™, requires sample preparation before analysis can be executed.

Drug discovery and clone selection
タンパク質特性評価
Media optimization
インプロセス発酵モニタリング

Using the OPUS RoboColumn on a Tecan Fluent liquid handling workstation generates sufficient purified product to conduct your analytical assays.

クロマトグラフィー

ATMP

Continuous Manufacturing

RGEN

Manufacturing Centers of Excellence

Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.

Locations

お客様第一。

サポートは、Repligenという企業の遺伝子に組み込まれています。弊社の目標は、卓越した顧客体験を提供すること、そしてRepligenの製品やサービスの適用や導入を効率よく成功に導くためにサポートすることです。

Field Application Support
カスタマーサービス
フィールドサービスエンジニア

お問い合わせ

仕様

プレートのサイズ	128.3 L x 86.0W x 14.0 mm H
プレートの素材	ポリオキシメチレン共重合体
穴の数	96
カラム容量	50 µL	100 µL	200 µL	450 µL	500 µL	600 µL
ベッド高さ	2.5 mm	5.0 mm	10.0 mm	22.5 mm	25.0 mm	30.0 mm
カラム内直径	5 mm
カラムの素材	ポリプロピレン
化学的安定性	一般的に使用されるすべての水性バッファー、pH 1〜14、有機溶剤**。ハロゲン化有機溶剤、ヘキサンは使用しないでください
カラム保存溶媒	AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride HIC, SEC, Affinity and MMC Columns: 20 % ethanol CHT, CFT Columns (Bio-Rad): 20 % ethanol + 10 mM sodium phosphate, pH 6.8
推奨保管温度	AIEC、CIEC、HIC、SEC、MMC: +4°C～+30°C アフィニティカラム: +2°C～+8°C
動作温度	+4°C～+30°C
最大動作圧力	最大8.0bar
流速	16～1000 cm/h**
ラベリング	各カラム本体にレーザーエッチング。ラベルの内容：製品番号樹脂名カラム容量（µL）

*注：充填したクロマトグラフィー樹脂の化学的安定性も考慮する必要があります。詳細については、メーカーの樹脂仕様を参照してください。

** TECAN software enables set up of the flow rate for each individual dispensing and chromatography step.

OPUS® RoboColumn® Pre-packed Columns

LEADER OF THE PACK

OPUS^®RoboColumn^® miniaturized pre-packed columns are designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.

OPUS RoboColumn Pre-packed Chromatography Colums