Chromatography Redesigned




Largest scale, broadest range

In response to the need to scale up chromatography processes, Repligen offers the largest pre-packed columns for the bioprocess industry. OPUS® 45R,  60R and 80R Columns are the first pre-packed columns designed to meet the chromatography requirements of larger 1000L and 2000L single-use bioreactors.

  • ベッドの高さや樹脂、用途に合わせて簡単に設定可能
  • Seamless scale-up from smaller OPUS® Columns
  • GMP使用向けの完全なドキュメントパッケージ
  • 従来のカラム技術と比較して、時間とコストを大幅に削減
  • Consistent materials of construction
  • Class VI, EMA 410/01 compliant materials
  • GMP and pre-GMP quality packages
  • ISO documentation (CoA, RSF)
  • Select from > 150 resins
  • Individual column release testing
 Ultimate Flexibility

Packed with user-specified resins

Repligen packs > 250 resins from 60 resin families >15 resin vendors. The most frequently packed resins are 30-120μm in particle size and based on matrices of agarose, methacrylate, cellulose, and polystyrene material.  A convenient resin pick-up service is available upon approval.

Full resin list




Repligen CaptivA® and CaptivA® HF
Repligen NGL COVID-19 Spike Protein AF Matrix
Repligen AVIPure® AAV2, AAV8 and AAV9
Purolite Praesto® AP/AP+, Jetted A50/A50+
Cytiva MabSelect™ Family
Cytiva AVB Sepharose™ HP
Cytiva Capto™ PlasmidSelect
Thermo POROS® CaptureSelect™ AAV/AF types
Thermo POROS® Heparin
Tosho TOYOPEARL® AF-rProtein A HC-650F


JSR Amsphere™ A3
Various Vendors IMAC/Chelating/Nickel
Purolite Praesto® (IEX)
Cytiva Capto™ ImpRes (IEX, HIC, MMC)
Cytiva Capto™ (IEX, HIC, MMC)
Cytiva Capto™ Core 400 and 700
Cytiva Capto™ S ImpAct
Cytiva Sepharose™ FF (IEX, HIC, MMC)
Cytiva Sepharose™ HP (IEX, HIC, MMC)
Cytiva SOURCE 30 (Q and S)
Cytiva Superdex (75 and 200)
Cytiva  Sephadex G25

ABT Rapid Run Agarose Bead (IEX)
Thermo POROS® 50 um (IEX, HIC)
Thermo POROS® 20 um (IEX)
Millipore Fractogel® (IEX)
Millipore Eshmuno® (IEX)
Sartorius HyperCel™ (PPA, HEA, CMM, MEP)
BioRad Nuvia™ (IEX, MMC)
BioRad Nuvia™ HP (IEX)
BioRad UNOsphere™ (IEX)
BioRad CHT™ Types: I & II, 40um, 80um, (≤60cm IDs)

Access CHT™ Resin pre-packed in OPUS Columns

Ceramic hydroxyapatite (CHT™) resin offers unique selectivity during the purification of mAbs and recombinant proteins. OPUS Columns can now be pre-packed with CHT™ resin and shipped world wide with excellent performance.

  • 内径5 - 45cm
  • ベッド高さ 5 - 30cm

View the full performance data




 Ultimate Flexibility

Innovative resin recovery port



With an innovative side port that allows for easy resin unpacking, OPUS 45R, 60R, and 80R Columns provide the ultimate flexibility in pre-packed column technology.

Without compromising  chromatographic performance or cleanability, the new feature helps mitigate the risk of implementing pre-packed columns in GMP settings, and allows for re-use of the unpacked resin in other columns.

In the event of a deviation, and if the OPUS pre-packed column performance cannot be recovered, the column can be unpacked and the resin can be repacked into a traditional, self-packed column. 
After the intended usage of the OPUS pre-packed column, the column can be unpacked and resin transferred to a development or pilot setting for additional use.




医療グレードのポリプロピレンホモポリマー製で、OPUS®カラムのすべての製品との接触面はEMA 410/01(または動物質不使用)でクラスVIに適合しています。

2.5 - 30 column tubeポリプロピレン
45R - 80Rカラムチューブ70% w/w Eガラス/PP工学的複合構造
インレット/アウトレット ポリプロピレン
充填層サポートスクリーン ポリプロピレン
Oリング プラチナ硬化医療グレードシリコン
戻り管路 強力なポリエステル繊維強化を施したプラチナ硬化医療グレードシリコン


リン酸還元の比色測定を用いたOPUS® 20カラムは6 FFで充填され、2 CVで残留リン酸を確実に洗浄できます。  

  • OPUS®カラムには大きなデッドスペースがありません
  • OPUS®は洗浄しやすい設計になっています



Impact on process economics

Substantial savings in manufacturing costs and labor are realized because OPUS Columns offer the benefits that come with pre-packed technology:

  • 先行資本支出不要
  • CIP手順とクロスコンタミネーションの削減
  • キャンペーンのターンアラウンド時間の短縮
  • 優れた運用性(労働要件の削減)

In this example, over 65% cost savings and up to 80% labor savings were achieved when using OPUS Columns compared to self-packed columns.*

*Assumptions: OPUS 60R Pre-packed Column versus purchase of new 60 cm diameter automated self-pack equipment (columns are dedicated to a specific molecule). Data presented are estimates and subject to change.

OPUS® 2.5 - 80R Pre-packed GMP-ready Columns

OPUS® Pre-packed Chromatography Columns provide linear scale-up from process validation to commercial and GMP manufacturing. Select from the broadest portfolio of pre-packed GMP-ready column sizes, including large scale OPUS® 45R, 60R, and 80R Columns. Pre-packed columns enhance facility efficiency overall by converting column packing resources into productive chromatography and accelerating campaign turnaround time.

Innovation in Purification

  • Configure bed height to nearest mm
  • Pack nearly any resin from nearly any supplier
  • Customize plat count, asymmetry and test method
  • Unpack resin using recovery port featured on OPUS® 45R, 60R and 80R columns
  • ISOクラス7のクリーンルームでパッキング
  • 詳細レギュラトリーサポートファイル
  • 各カラム構成の分析証明書

OPUS GMP manufacturing process 

Click on the links to explore different features.

再生アイコン 動画ポスター
The OPUS® time machine

OPUS® Columns pack your choice of resin and the most precious element of all, time, into a self-packed ready-to-use chromatography column at almost any size and for almost every application. Skip months of hardware validation, execute more runs--and still leave work early to enjoy a bike ride. Don’t worry, we won’t tell your boss.

再生アイコン 動画ポスター
Small but mighty

Reallocate column packing time and resources toward productive GMP-level chromatography, even at small-scale. The family of OPUS® Pre-packed Columns now includes the small but mighty OPUS® 2.5. Scale GMP purification effortlessly from 2.5 cm to 80 cm ID.

Manufacturing Centers of Excellence

Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.




  • Field Application Support
  • カスタマーサービス
  • フィールドサービスエンジニア