LONG® R³ IGF-I細胞培養サプリメント
細胞培養において、インスリンよりも200倍強力で、2倍安定しているLONG®R3 IGF-I（LONG R3）は、ヒトインスリン様成長因子-I（IGF-I）の組換え類似体で、主として細胞培養パフォーマンスの向上のために開発されました。
ドライタイプと液体タイプが複数の細胞腫で利用可能。
Animal-free manufacturing process
Comprehensive supply chain risk management
HIGH PERFORMANCE
More Potent than Insulin
LONG® R3 IGF-I is a recombinant analog of human insulin-like growth factor-I (IGF-I) specifically engineered to enhance cell culture performance.
- インスリンやネイティブIGF-Iよりも生物学的に効果のあるインビトロ
- Ideal for systems utilizing serum-free or low-level serum applications
- Increased cell density, higher viability and extended culture duration
Request a sample today and boost your cell productivity
Try a sample of LONG R3-IGF-I in your cell culture process to see how it can enhance cell performance and productivity. Start improving your cell performance today.
HIGH PERFORMANCE
Better science for better cell culture
LONG®テクノロジーは、成長ホルモン配列の一部と成長因子を組み合わせたものです。この場合、成長因子の生物活性を移すことなく、成長因子の生産を改善できます。
明確な生物学的利点
All mammalian cells secrete IGF Binding Proteins (IGFBPs) which bind to and inhibit native IGF-I. The substitution of an arginine for glutamic acid at position three in LONG® R3 IGF-I, in conjunction with the 13 amino acid N-terminal extension peptide, results in > 1000 fold reduced affinity for IGFBPs, enhancing its bio-availability and effectiveness in comparison to native IGF-I.
特異性は生産性向上の鍵
Unlike insulin, LONG® R3 IGF-I specifically targets and activates the type I IGF Receptor (IGF-IR) resulting in greater activation of downstream signaling cascades responsible for proliferation and inhibiting apoptosis. Greater activation of these key pathways results in increased cell density, higher viability and extended cell culture productivity.
To learn more about the use of LONG® R3 IGF-I in stem cell and cell therapy applications, please contact Repligen.
細胞培養の最適化
生産性
Cell culture supplements are essential for long-term growth and productivity of cell lines in serum-free media formulations. Both insulin and LONG® R3 IGF-I, a more potent supplement that directly targets and activates the IGF-I receptor, are employed by the biopharmaceutical industry today to support the growth of recombinant cell lines. This webinar will show how these two supplements, used separately and in combination, impact viable cell density and IgG production of multiple CHO cell lines. It will demonstrate how a combination of LONG® R3 IGF-I and insulin results in less protein supplementation and, therefore, less potential impact on downstream processing.
HIGH PERFORMANCE
Improved volumetric productivity
LONG® R3 IGF-I has been shown to improve volumetric productivity in CHO cell culture by up to 62% over cell culture with no supplementation and 40% over cell culture supplemented with insulin.
LONG® R3 IGF-I Cell Culture Supplement
LONG® R3 IGF-I is a critically important mitogenic growth factor often used in mammalian cell culture to increase yield significantly. Such growth factors are fundamental supplements to chemically-defined media.
LONG® R3 IGF-I is compatible with multiple cell types. Any mammalian cell type that responds to insulin in cell culture has the potential to respond to LONG® R3 IGF-I.
- CHOクローン
- HEK 293
- Vero
- MDCK
- 線維芽細胞
LONG® R3 IGF-I is available in two formats, both stable at 2-8°C.
LONG® R3 IGF-I can also be milled into cell culture media for additional flexibility and convenience.
Since LONG® R3 IGF-I ships at ambient conditions and stored at 2-8°C, cold chain shipping and storage costs can be significantly reduced.
フリーズドライパウダー
- 5 mL（サンプルサイズ*）
- 50 mg
すぐに使える液体タイプ
- 5 mL（サンプルサイズ*）
- 100 mL
- 250 mL
- 500 mL
- 1L (1000 mL)
*サンプルサイズは完全には検証されていません。サンプルは、検証済みの中間原料から充填されます。安定性に関する情報は、100 mL（液体タイプ）と50 mg（パウダー）の安定性試験から推測されたものです。
LONG® R3 IGF-I ELISA Kit
The only commercially available kit for the quantitation of LONG® R3 IGF-I, the kit is fast, accurate, and easy to use with both cell culture and drug substance samples. The sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
- ネイティブおよび組換えIGF-1に対する反応性は限定的
- インスリンに対する反応性なし
- すぐに使えるELISAプレートなら、追加のプレートコーティングやブロッキングのステップなしで、すぐにアッセイを実行できます
キットの内容：
- LONG® R3 IGF-I coated strip plate, 96 wells
- LONG® R3 IGF-I Standard
- 希釈剤
- ビオチン標識した検出抗体
- ストレプトアビジン-ペルオキシダーゼ
- TMBペルオキシダーゼ基質
- LoBind® 1.5mL エッペンドルフチューブ
ソリューション
Manufacturing Centers of Excellence
Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。
Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.
お客様第一。
サポートは、Repligenという企業の遺伝子に組み込まれています。弊社の目標は、卓越した顧客体験を提供すること、そしてRepligenの製品やサービスの適用や導入を効率よく成功に導くためにサポートすることです。
- Field Application Support
- カスタマーサービス
- フィールドサービスエンジニア
Related products
LONG R3 IGF-I ELISA Kit
The LONG® R3 IGF-I ELISA Kit is the only commercially available kit for the quantitation of LONG® R3 IGF-I. Fast, accurate, and easy to use with both cell culture and drug substance samples, this sandwich ELISA kit uses a biotinylated secondary antibody and Streptavidin-HRP for detection.
リソース
