KrosFlo® TFDF®システム
AAV、レンチウイルス、腫瘍溶解性療法、レトロウイルス、アデノウイルス、エクソソーム、およびマイクロキャリア上の細胞に最適な、上流のウイルスベクターバイオプロセシングを簡素化および強化する画期的なTFDF®（タンジェンシャルフローデプスフィルトレーション）テクノロジー。
Intensification Starts Here!
Discover how KrosFlo® TFDF® based process intensification leads to high virus production combined with simplified clarification compared to batch cell culture.
- Implement TFDF® to mitigate cell density effects
- TFDF® facilitates intensified production of lentivirus and AAV
- Simplify clarification techniques to streamline your production process
INNOVATION BREAKTHROUGH
TF + DF = TFDF® Combined Benefits
タンジェンシャルフロー
高い細胞密度
不十分な製品通過
デプスフィルトレーション
低い細胞密度
十分な製品通過
タンジェンシャルフローデプスフィルトレーション
高い細胞密度
十分な製品通過
INNOVATION BREAKTHROUGH
Novel TFDF® Filter
The thick-walled 2 - 5 µM tubular filter captures cells and cell debris with reduced fouling and negligible product retention. The feed stream enters the bottom port. The retentate travels through the lumen and exits at the top of the tube. The permeate exits the enclosure through the lower permeate port.solution. The combination of tangential flow and depth filtration in one filter produces a high-flux, high-capacity solution. h
INNOVATION BREAKTHROUGH
相乗効果
- Low concentration polarization minimizes fouling
- Short, highly inter-connected paths do not retain product
- 小さな設置面積
- 2-5 micron pore size for large biomolecules
INTEGRATED
Filter, Flow Path and System as One Solution
KrosFlo® TFDF® Filter
ProConnex® TFDF® Filter Assembly
KrosFlo® TFDF® Systems
INTEGRATED
Complete, Ready-to-use TFDF® Filter Assemblies
Single-use, Gamma-irradiated and closed ProConnex® TFDF® Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state. ProConnex® TFDF® Flow Paths are complete assemblies that include the TFDF® Filter, a disposable pump head, integrated sensors, tubing and aseptic connectors.
TFDF® Filter
Surface Area: 3 cm2
Surface Area: 30 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 3 cm2
Surface Area: 30 cm2
Surface Area: 50 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 150 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 450 cm2
Surface Area: 750 cm2
Surface Area: 1500 cm2
Surface Area: 2100 cm2
ProConnex® TFDF®
Flow Path
Surface Area: 6000 cm2
Industrial grade components and extended automation allow for confident process scaling. With the same software, hardware, controls and cGMP compliance built into every system, process change challenges in scale-up are removed.
READY TO SCALE FAST
Maintain processes while scaling to production
The KrosFlo® TFDF® System delivers application-specific process control logic for optimal TFDF process performance. Systems scale from 1 L to 2000 L in a small footprint of 1 m2 for both pilot (31 inch x 42 inch) and process (37 inch x 42 inch) systems.
KrosFlo® TFDF Lab System
1 - 50 L volume
3 - 160 cm2 surface area
KrosFlo® TFDF® Pilot System
1 - 500 L volume
500 - 1600 cm2 surface area
KrosFlo® TFDF® Process System
50 - 2000L volume
3000 - 6000 cm2 surface area
READY TO SCALE FAST
Capacity increases with tube number and length
1～2000 Lのリニアスケーラビリティを実現するには、エンクロージャーごとのチューブの長さとチューブの数を増やします。チューブ長さは20 cmから108 cmまで増やせます。フィルターの有効長さは、フィルターの物理的長さより短くなることがあります。エンクロージャーごとのチューブ数は、1から40まで増やせます。
チューブの数
チューブ1本
表面積：3 cm2
推奨容量：<1 L
チューブ1本
表面積：150 cm2
推奨容量：<50 L
チューブ10本
表面積：1500 cm2
推奨容量：<500 L
チューブ40本
表面積：6000 cm2
推奨容量：<2000 L
* additional sizes available
チューブ長さ
物理的長さ：20 cm
有効長さ：2 cm
表面積：3 cm2
物理的長さ：108 cm
有効長さ：108 cm
表面積：150 cm2
KrosFlo® TFDF®システム
The KrosFlo® TFDF® System delivers a complete solution for optimized tangential flow depth filtration, with integrated process control logic and a single-use, Gamma-irradiated ProConnex® TFDF® Flow Path with integrated sensors.
Click on the tabs to explore features of the different KrosFlo® TFDF® Systems and Flow Paths.
KrosFlo® TFDF®ラボシステム
容量 1 - 50L
表面積 3- 160 cm2
Click on the links to explore different features.
KrosFlo® TFDF® Pilot System
容量 1 - 500L
表面積 500- 1600 cm2
Click on the links to explore different features.
KrosFlo® TFDF® Process System
容量 50 - 2000L
表面積 3000- 6000 cm2
Click on the links to explore different features.
ProConnex® TFDF® Flow Path
Click on the links to explore different features.
KrosFlo TFDF Lab Flow Paths
Off-the shelf, single-use, gamma-irradiated and closed TFDF Lab Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.
Custom KrosFlo TFDF Pilot and Process Flow Paths
When scaling up, configure a flow path to meet your process requirements for your pilot and process KrosFlo TFDF Systems. All KrosFlo TFDF flow paths are single-use, gamma-irradiated and arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.
Replace sluggish messy depth filtration with combined tangential flow and depth filtration. No need to load multiple filters or hunt for parts. At 1L through 2000L, connect one fully integrated filter module and go -- at flux speeds up to 1950 LMH.
2020-12-14
01:35
If you have three minutes, then you have enough time to install a TFDF® flow path. Mount the device, make a few connections and you’re done - without ever breaking a sweat.
2021-02-25
01:57
Manufacturing Centers of Excellence
Repligenは、ISO 9001品質管理システムに基づいて、バイオ医薬品業界向けの製品を開発・製造しています。高品質で安定した、堅牢な製品をタイムリーに提供し、お客様の事業継続性を保証することを重視しています。
Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.
Expert Project Management
お客様第一。
サポートは、Repligenという企業の遺伝子に組み込まれています。弊社の目標は、卓越した顧客体験を提供すること、そしてRepligenの製品やサービスの適用や導入を効率よく成功に導くためにサポートすることです。
- Field Application Support
- カスタマーサービス
- フィールドサービスエンジニア
関連製品
ProConnex® Flow Paths for Systems
Expertly designed plug-and-play flow path
ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility.
リソース
Process intensification for lentiviral vector manufacturing using tangential flow depth filtration
by Robert M. Tona, Reeti Shah, Kimberly Middaugh, Justin Steve, João Marques, Blair R. Roszell, Cindy Jung
Cell Press, March 2, 2023
Production of Lentiviral Vectors Using a HEK-293 Producer Cell Line and Advanced Perfusion Processing
by Michelle Yen Tran and Amine A. Kamen
Frontiers, June 14, 2022
Lentiviral vector manufacturing process enchancement utilizing TFDF® technology
Thomas Williams、Oliver Goodyear、Lee Davies、Carol Knevelman、Michael Bransby、Kyriacos Mitrophanous、James Miskin
Cell and Gene Therapy Insights、2020年4月
